Obtinerea polizaharidelor functionalizate pentru aplicatii in biomedicina si biotehnologii

Data ultimei actualizări : 06.12.2016 | Tipăreşte pagina

1) Grant number : PN-II-PT-PCCA-2011-3.1-087

2) Scientific authority: Unitatea Executivă pentru Finanţarea Învăţământului Superior, a Cercetării, Dezvoltării şi Inovării (UEFISCDI)

3) Grant title: Obtinerea polizaharidelor functionalizate pentru aplicatii in biomedicina si biotehnologii

4) Partners: Institutul National de C-D in Electrochimie si Materie Condensata Timisoara

5) General objectives -The general objective of the BIOPOLIZAH project is to develop original and highly efficient strategies to allow functionalization (at preparative scale) of long chain, polydisperse mixtures of polysaccharides with functional groups such as aromatic amines, oligopeptides and chromophores, followed by detailed analysis of the coupling products using the most advanced glycoscreening and sequencing methods based on high performance mass spectrometry (MS), microfluidics technology and robotics for sample infusion into MS.

6) Grant results:

Articles

• 1) Zamfir a.D.; Fabris, D.; Capitan, F.; et al., Profiling and sequence analysis of gangliosides in human astrocytoma by high-resolution mass spectrometry., Anal. Bioanal.Chemistry, 2013, 405 (23) 7321-7335 
•  2) 1.R.M.Ghiulai,M.Galusca,I.Sisu,E.Sisu,A.D.Zamfir, High resolution mass spectrometric characterization of amino linked oligosaccharides - a preliminary study, Cent. Eur. J. Chem. 2013,11(8),1309-1319
• 3) Flangea, Corina; Fabris, Dragana; Vukelic, Zeljka; et al., Mass Spectrometry of Gangliosides from Human Sensory and Motor Cortex, Auatralian Journal of Chemistry , 2013,66 (7),781-790   
• 4) C.Bolcu, G.Vlase, T.Vlase, P.Albu, N.Doca, E.Şisu, Thermal Behaviour of some polyuretanes reticulated by aminated maltose
  J.Thermal.Analysis , 2013, 113, 1409–1414

Chapters in books

• 4)Fructooligosaccharides and heterooligofructans. Carbohydrate Chemistry: Chemical and Biological Approaches, RSC-Royal Soc. Chem. Publishing (book chapter) U.K., July 2012, 38, 176-191.

Communications

• 5. I.Sisu,A.Serb,R.Ghiulai,M.Penescu, S. Dragan, A.D.Zamfir, E.Sisu-Structural analysis of functionalized oligo and polysaccharides by mass spectrometry -XXXII-nd Romanian Chemistry Conference, October 3-5, 2012 Calimanesti-Caciulata, Valcea,Romania
• 6. Al.Serb, S.Dragan,C.Flangea, R.Ghiulai,M.Penescu,E.Sisu,Al.D.Zamfir Analysis by mass spectrometry of the oligosaccharides from natural glycoconjugates XXXII-nd Romanian Chemistry Conference, October 3-5, 2012 Calimanesti-Caciulata, Valcea, Romania
• 7. C.Flangea,C.Schiopu, F.Capitan, D.Seidler, E.Sisu,Alina D. Zamfir Determination of decorin chondroitin/dermatan sulfate expression in human embryonic kidney cells by fully automated chip-based nanoelectrospray mass spectrometry- International Simposium Research and Education in Innovation Era, 8-9 Nov. 2012, Arad, Romania
• 8. C. Flangea, D.G. Seidler, E. Sisu, A.D. Zamfir Identification of unusually sulfated tetra- and hexasaccharides in mouse brain proteoglycans by chip-nanoelectrospray collision-induced dissociation ms2-ms4 - 6th Central ans Eastern European Proteomic Conference 14-17th October 2012, Budapest , Hungary
• 9. C. Mosoarca, I. Malaescu, T. Visnapuu, T. Alamäe, A.D. Zamfir Chip-based nanoelectrospray mass spectrometry analysis of fructooligosaccharides produced by levansucrases from Pseudomonas syringae and aurantiaca - 6th Central ans Eastern European Proteomic Conference 14-17th October 2012, Budapest , Hungary
• 10. Şişu,R.Ghiulai,A.Lascu, A.Şerb, F.Capitan, M.C.Pascariu, L.M.Rusnac, E. Şişu. Mass
  spectrometry analysis of maltodextrin-lactones - Zilele Academice Timisene, ed. XIII- Noi tendinte si strategii în chimia materialelor avansate cu relevantă în sisteme biologice, tehnică si protectia mediului 13-14 iunie 2013, Timisoara,Romania
• 11. C.Pascariu, A. Gruia, C. Neanu, M.Rafaila, L.M.Rusnac, E.N.V. Sisu - Structure determination of sugar monStructure mono- and diacetal derivatives through GC-EI-MS combined with computational chemistry - Zilele Academice Timisene, ed. XIII- Noi tendinte si strategii în chimia materialelor avansate cu relevantă în sisteme biologice, tehnică si protectia mediului 13-14 iunie 2013, Timisoara, Romania
• 12. A.F.Serb, Z.Vukelic, C.Flangea,C.Schiopu, E.Sisu, A.D.Zamfir- Investigation of Oligosaccharides from Biological Matrices Using Modern Mass Spectrometric Methods - A Preliminary Study- Zilele Academice Timisene, ed. XIII- Noi tendinte si strategii în chimia materialelor avansate cu relevantă în sisteme biologice, tehnică si protectia mediului 13-14 iunie 2013, Timisoara, Romania
• 13. C.Schiopu,F.Capitan,Z.Vukelic,E.Sisu,A.Zamfir – Deciphering the composition and structure of glicolipids in human meningioma by advanced mass spextrometry-Zilele Academice Timisene, ed. XIII-Sectiunea medicina, 23 Mai,2013,Timisoara,Romania
• 14. Roxana M. Ghiulai, Mirela Sarbu, Ioana Sisu, Eugen Sisu, Alina D. Zamfir
  QTOF TANDEM MS FOR THE ANALYSIS OF DERIVATIZED AND UNDERIVATIZED OLIGOSACCHARIDES , 2nd Middle Eastern and Mediterranean Sea Region Countries  Mass Spectrometry Conference, 30 iunie-4  iulie, 2013, Siena, Italy

2015
ARTICOLE

• 1) Ciucanu I. ,Pilat L., Ciucanu C.I. ,Sisu E., Determination of Neutral Monosaccharides as Per-O-methylated Derivatives Directly from a Drop of Whole Blood by Gas Chromatography-Mass Spectrometry, Analytical Chemistry - 2015,87,21,10856-10861
• 2) Sarbu M., Zhu F.F., Peter-Katalinic J., Clemmer D.E., Zamfir A.D., Application of ion mobility tandem mass spectrometry to compositional and structural analysis of glycopeptides extracted from the urine of a patient diagnosed with Schindler disease, Rapid Communications in Mass Spectrometry - 2015,29,21,1929-1937 
• 3) Capitan, F ; Robu, AC ; Schiopu, C ; Ilie, C ; Chait, BT ; Przybylski, M ; Zamfir A.D, beta-Lactoglobulin detected in human milk forms noncovalent complexes with maltooligosaccharides as revealed by chip-nanoelectrospray high-resolution tandem mass spectrometry, Amino Acids-2015,47,11,2399-2407
• 4) Robu, AC; Popescu, L ; Munteanu, CVA  ;Seidler, DG ; Zamfir A.D, Orbitrap mass spectrometry characterization of hybrid chondroitin/dermatan sulfate hexasaccharide domains expressed in brain, Analytical Biochemistry-2015,485,122-131;
• 5) Capitan, F ; Robu, AC ; Popescu, L ; Flangea, C; Vukelic, Z ; Zamfir A.D, B Subunit Monomers of Cholera Toxin Bind G1 Ganglioside Class as Revealed by Chip-Nanoelectrospray Multistage Mass Spectrometry, Journal of Carbohydrate Chemistry-2015,34,7,388-408 
• 6) Sarbu, M ; Munteanu, CVA ; Dehelean, L ; Petrescu, AJ ; Peter-Katalinic, J ; Zamfir A.D, Identification and structural characterization of novel O- and N-glycoforms in the urine of a Schindler disease patient by Orbitrap mass spectrometry, Journal of Mass Spectrometry-2015,50,9,1044-1056
• 7) Pascariu M.C., Niculescu M., Belengeanu D., Serb A., Bozin L.A., Mitar C.M., Ciopanoiu  I.R., Lupea A.X., Radical Cations in EI-MS Analysis of Drugs. 1. Riboflavin, Epinephrine, Chloramphenicol, Metronidazole and Dipyridamole, Revista de  Chimie-Bucharest, 2015, 66(10), 1582-1589
• 8) Pascariu M.C., Ciopanoiu I.R., Mitar C.M., Mracec M., Sisu E., Exploring the possibility of anchimeric assistance in alkyl sugar cations by computational methods, Revue Roumaine de Chimie., 2015, 60, 881-889
• 9).L.Mocanu ,D.Ivan, M. Schulz, M. Mracec, S. Funar-Timofei, M.Mracec, M. Medeleanu, E. Sisu, Ab Initio Conformational Analysis of the (3S, 5R, 6R)-6 Acetylamidopenicillanic Acid, International Journal of Advanced Research in Chemical Science (IJARCS)-2015,2, 10, 26-41;

COMUNICARI

Strainatate

• 1) Bozin L., Neanu C., Pascariu M.C., Belengeanu D., Dragomirescu A., Serb A., Peter F., Sisu E.,TLC/densitometry analysis of diastereomer mixtures as pseudo-ceramide precursors for dermato-cosmetology, 17^th DKMT Euroregional Conference on Environment and Health, 5-6 iunie 2015, Szeged, Ungaria,
• 2) Serb A.F., Bozin L., Dema A., Georgescu M., Sisu E.,Zamfir A.D, Analysis of glycoconjugates from primary brain tumors using a complex mass spectrometric methodology, 9^th International Conference on Instrumental Methods of Analysis: Modern Trends and Applications - IMA 2015, 20-24 September 2015,   Kalamata, Grecia
• 3) Pascariu M.C., Bozin L.A., Serb A., Dinca N., Sisu E., Semi empirical proof of long bond states as intermediates in mass spectrometry fragmentation of aldohexofuranose derivates, 21^st International symposium on analytical and environmental problems, 28 September 2015, Szeged, Ungaria
• 4) Bozin L.A., Dragomirescu A., Simu G., Pascariu M.C., Serb A., Sisu E., TLC- Densitometric investigation of bioactive components from Mediterranean algae, 21^st International symposium on analytical and environmental problems, 28 September 2015, Szeged, Ungaria,

Tara

• 5) Pascariu M.C., Niculescu M., Serb A., Bozin L., Sisu E., Radical cations in EI-MS analysis of drugs. 1. Riboflavin, The Academic Days of Arad, 25^th edition, 15-17 mai 2015, Arad, România,
• 6) Pascariu M.C., Rusnac L.M., Medeleanu M., Sisu E., Neighboring group participation in halocarbohydrate derivatives as the pathway

LISTA LUCRARI 2016

 Articole publicate

• 1.Ciucanu I.,Pilat L., Ciucanu C.I.,Şişu  E., Dumitraşcu V, Simultaneous analysis of neutral monosaccharides, fatty acids and cholesterol as biomarkers from a drop of blood., Bioanalysis, 2016, 8(20),2147-56.
• 2.Zamfir A.D., Applications of capillary electrophoresis electrospray ionization mass spectrometry in glycosaminoglycan analysis, ELECTROPHORESIS,2016,37(7-8), 973-986
• 3.Sarbu M. , Robu A.C.,  Ghiulai R.M.,  Vukelic Z,  Clemmer D.E., Zamfir A.D., Electrospray Ionization Ion Mobility Mass Spectrometry of Human Brain Gangliosides, ANALYTICAL CHEMISTRY,2016,88(10) 5166-5178
•  4.Robu A.C.,  Vukelic Z.,  Schiopu C., Capitan. F, Zamfir A.D., Mass spectrometry of gangliosides in extracranial tumors: Application to adrenal neuroblastoma, ANALYTICAL BIOCHEMISTRY,2016, 509, 1-11
• 5.Pascariu M.C.,Rafaila M., Medeleanu M., Badea V.,Gruia A., Purcarea V., Penescu M., Sisu E., Computational search for long bonds in radical cations of sugars. 1. Glucofuranose, galactofuranose and mannofuranose di-O-isopropylidene derivatives, FARMACIA, 2016, 64(4), 553-564,
• 6. Medeleanu M., Olariu T.,Georgescu D.Sisu E.,Mracec M.,Mracec M., Conformational analysis and formation enthalpy of (3r,5s,6r)-6-acetylamido penicillanic acid calculated by the PM3, PM and PM7 semiempirical MO methods, Rev. Roum. Chim. 2016, 61(4-5), 445-458

COMUNICARI

STRAINATATE

• 1) Bozin L,. Pascariu M.C., Dragomirescu A., Simu G., Dinca N., Sisu E., Characterization by soft ionization mass spectrometry methods of mono- and oligosaccharides functionalized at the anomeric center in  Proceedings of The 22nd International Symposium on Analytical and Environmental Problems, 10 octombrie 2016, Szeged, Ungaria p.435-438, ISBN 978-963-306-507-5, 22nd International Symposiumon Analytical and Environmental Problems, October 10, 2016, Szeged, Ungaria
• 2) Simu G., Dragomirescu A., Andoni M., Bozin L., Sisu E., Use of some natural supports for the adsorption of theophylline from aqueous solutions in center in  Proceedings of The 22nd International Symposium on Analytical and Environmental Problems, 10 octombrie 2016, Szeged, Ungaria p.285-288, ISBN 978-963-306-507-5, 22nd International Symposiumon Analytical and Environmental Problems, October 10, 2016, Szeged, Ungaria
• 3) Pascariu M.C., Rafaila M., Medeleanu M., Curpan R., Pacureanu L., Dinca N., Sisu E., DFT study of long bonds in radical cations of sugar derivatives, in center in  Proceedings of The 22nd International Symposium on Analytical and Environmental Problems, 10 octombrie 2016, Szeged, Ungaria p.251-254, ISBN 978-963-306-507-5, 22nd International Symposiumon Analytical and Environmental Problems, October 10, 2016, Szeged, Ungaria

IN TARA

• 4) Georgescu A., Bozin L., Georgescu M., Dema A., Pascariu M.C., Sisu E., Assessment of distribution of glycoconjugates obtained from natural matrices, The 12th national congress of the romanian society of physiological sciences, 26-28 mai 2016, Craiova, Romania
• 5) Medeleanu M., Pascariu M.C., Sisu E., Mracec M., Mracec M., Conformer entropy influence on the biological activity of bioactive molecules, 16th International Conference of Physical Chemistry – ROMPHYSCHEM-16, 21-24 septembrie 2016, Galaţi, România
• 6) Dinca N., Pascariu M.C., Georgescu A., Bozin L., Simu G., Sisu E., Qualitative chemical analysis possibilities usingdifferential mass spectrometry and quantum chemical calculations, A XXXIV-a Conferinţă Naţionala de Chimie, 4-7 octombrie 2016, Căciulata, România
• 7) Sisu I., Bozin L., Pascariu M.C., Dinca N., Georgescu A., Dragomirescu A., Sisu E., ESI-IT-MS  characterization  of  amino  acids  functionalized with oligosaccharide derivatives, A XXXIV-a Conferinţă Naţionala de Chimie, 4-7 octombrie 2016, Căciulata, România
• 8) Pascariu M.C., Niculescu M., Medeleanu M., Mracec M., Dincă N., Sisu E., DFT study of anchimeric assistance in some glycoderivatives, Molecular Modeling in Chemistry and Biochemistry Conference - MolMod 2016, 13-15 noiembrie 2016, Cluj-Napoca, România